Germ Cell Cancer – How To Prove The Crenation Test Solution?
Germ Cell Cancer – Crenation Test
Germ cell cancer – From the first edition of this work, it became evident that several workers found it difficult to achieve results with the crenation test. They found their cells all failing to crenate to a certain degree. For instance, one doctor brought his solution to me. I used it for taking a sample of his own blood, while I used the same puncture to take a test with my own solution. Both were mounted on the same counting chamber; his solution showed a lack of crenation throughout the fields while mine showed a perfect crenation picture throughout.
Germ Cell Cancer – How To Prove The Crenation Test Solution?
This experiment has called for this additional instruction. Either his sodium chloride was faulty, or his solution imperfectly measured out. So to make sure your solution is exactly right and sufficiently strong to insure total crenation of all healthy cells, make or have made up for your use a pure solution consisting of a full and exact 1 % solution of sodium chloride, C.P. With this solution at hand, select some young and extremely healthy person who is willing to give a few drops of blood from the lobe of the ear. (It hurts less here and is easier to squeeze the blood out than it is from the finger tip). Now make your puncture, draw your blood up to the .5 mark on your red diluting pipette, and immediately complete filling the pipette from your NaCl 1% solution. Immediately shake it well to insure perfect distribution of the blood; then, as soon as practicable, mount it on the counting chamber and observe it under the microscope. This should be done within five to ten minutes after the blood is drawn.
If your solution is exactly right, every cell in the microscopic field will be fully crenated by the time the cells have settled down ready to start counting. If they show unevenness in the process of crenation, some shriveled and others half shriveled, and others more flat than crenated, then your solution is not up to standard. In this event dip the tip of your knife in your pure salt and add to the bottle a few salt crystals that can be picked up on 4mm. of the knife blade tip. Again try the solution. Indeed, keep doing this until you succeed; for it will work, and it is safer to have a strong solution than to have a weak one. A solution that is too strong may cause a breaking and dissolving of the weak cells, but it will crenate them first. So it is important to start with as nearly a 1% solution as is possible, and add only tiny amounts until it works perfectly. The effort to do this will be well worth while. Now you are ready to test any person’s blood for cancer. 5% or more means growth.
Germ cell cancer – Good culture medium
With this crenation problem licked, the next item is to secure a good culture medium. Here again I must draw upon memory after over twenty years. It runs in my mind that we used a neutral glucose broth, using about 20 cc’s to the tube, and into each tube was added about one gram of calf’s brain. This was doubly sterilized. That is, it was first sterilized, and then after a wait of forty-eight hours at room temperature, it was again sterilized. This to check any unresponsive spore that might by chance be in the brain tissue.
It is easy enough to check the effectiveness of this formula. Any cancerous person, with internal or external cancer, can supply the necessary five cc’s of blood from which to make a culture. Draw the blood very carefully from a thoroughly sterilized venipuncture, put it in the tube and incubate not less than six days unless positive growth is observed before.
When positive growth is observed, and it will be seen if the person has germ cell cancer and if the culture media is correct, then plate it out on blood-agar plates and incubate. Growths will be observed as tiny white colonies along the streaks within twenty-four hours. These will be easily identified by using a magnifying glass as of two structural varieties, one the tiny smooth and round colony, the other a round but rather than being smooth it will have tiny rootlets striking out from its outer margins.
The rough edged, rooted colonies, when stained, will be found to be formed by GLOVER’S BACILLUS.
Germ cell cancer – Nabel’s organisms
The smooth, round colonies, when stained, will be found to be formed by NABEL’S ORGANISMS.
When this point has been reached, the next item will be to focus on accomplishing two things: one will be the formation of a potent toxoid which will, when administered to a cancer patient, cause the body to produce antibodies and thus to throw off the cancer infection. With the infection killed, the growths will be reabsorbed spontaneously, or they can be cut out should the patient be in a hurry. The second item is to find a bacteriophage which can be used to sweep all the germ cell cancer from their natural habitat, which is the bowels of the victim where they live as sapprophytes, or eaters of filth, especially of decomposing protein ele-ments being thrown off because too much of it is being introduced to supply the body economy.
For the making of the toxoid, the procedure is well known to every able bacteriologist. The process to be followed is identical to that followed in making the toxoid for diphtheria. In my own experience every patient to which this toxoid we had was given made a complete recovery and did it rapidly. Mrs. Doty whose picture is reproduced herein happens to be one of the first of such victims to which the toxoid was administered. She is alive and well today after over twenty years of wonderful health. You can read about it at How to cure cancer?.
Germ Cell Cancer – Making of the bacteriophage
The making of the bacteriophage can be accomplished easily. Get your growth well developed on your blood agar platelets, or such other artificial media as you may, by experimentation, decide to use. Now find some hospital sewage disposal outlet, preferably the outflow from a septic tank. If no hospital septic out-flow is available, then go to the city septic tank out-flow. Secure some of the blue mud that forms at the existing place. Dip it up in a clean container and take it to your laboratory.
Germ cell cancer – Here is the important part
Now comes the important part. Using the utmost caution not to expose your plates to other contamination, burn your platinum loop and then dip it into the sludge from the septic tank outlet. Carefully lift the Petrie dish cover and streak once across the plate with that dipping. Re-burn and from another spot in the sludge streak again. Continue this until the entire plate is covered with streaks a quarter of an inch apart. When completed, place the plate in the incubator.
If you are successful the first time, when you examine that plate the next morning, you will find tiny spots of clearness appearing on the streak, as if the growth were turning to water. Pick out the largest of these spots, dip into it your freshly burned platinum loop, and carefully transfer this to a fresh plate of germ cell cancer growth as a series of enveloping circles. This done, recover and put to incubate.
If you have not gotten any contamination, when you re-examine this incubated plate, you will find the cancer growth either in the process of being eaten up, or already eaten up within the circle of your impregnation. This is because you have isolated a true bacteriophage, which is nothing else than a germ which lives by eating up another germ.
Wash off this cultured phage after it has destroyed every vestige of the cancer colony growth and one cc of the solution swallowed even highly diluted will sweep every germ cell cancer from the human cesspool . . . the digestive tract … of an inflicted human. Indeed, it may even be absorbed into the blood; this is something yet to be further developed.
When this information and practice becomes universal, germ cell cancer will be as effectively controlled as is today the disease of DIPHTHERIA.
